Sitemap
A list of all the posts and pages found on the site. For you robots out there is an XML version available for digesting as well.
Pages
Posts
My Chai
Published:
Malabar Chai has been my go-to beverage for a while and this is a fool-proof recipe I use to bring back the Malabar Chais that have gotten me through my Masters thesis in India.
Music performances
Published:
I have been learning to sing carnatic music since I was in primary school. Following a newfound appreciation for the art around 2018 as an undergrad student in Bangalore, I renewed classes and have been enjoying listening, singing and taking the opportunity to perform at various local events and contests. Here are a few videos of my performances in the past few years:
Eggplant Fry - South Indian Style
Published:
This is a simple recipe for a delicious South Indian style Eggplant (aka Brinjal, aka Katthirikkaai) fry. While it is a great side dish to pair with various South Indian dishes it also works a great way to incorporate eggplants in a meal as side with rice or bread. I have been told by friends who were not fans of eggplants that they enjoyed this dish because of all the other flavors and components that make up the dish. While there are several variations and recipes and videos for authentic versions of this dish, I have adapted this recipe to be simple and accessible to those with a kitchen not particularly equipped for South Indian cooking without compromising its key flavors!
engagement
Outreach to highschool students
Published:
Talked about synthetic biology and the iGEM competition to highschool students in Vidya Mandir school, Chennai, India
iGEM India BioSummit
Published:
Moderated the discussion on Health & Medical Data Privacy built around the sustainable development goals Industry, Innovation, and Infra (Goal 9), Good health and Well Being (Goal 3) at the iGEM India BIOSUMMIT 2020
Decolonizing North-South Research Collaboration Workshop
Published:
Participated in a workshop exploring the challenges and opportunities in North-South research collaboration. During the discussions we identify the barriers to equitable research collaboration and develop strategies to overcome them. I contributed to discussions on power dynamics, knowledge production, and capacity building in research partnerships.
Contribution to the scientific community
Published:
Overview of contributions to the scientific community in the form of peer review, committee participation, and contribution to the develpment of community standards.
research
Apis species comparison
Published:
Honeybees serve as great model for studying gut microbiota evolution in the context of host ecology and evolution given their well-documented ecology and evolutionary history. Unlike the more diverse microbiomes of humans, primates, and mice, honeybees offer a more tractable system for understanding how gut microbiota are distributed and have evolved. This research is crucial for understanding how symbiotic interactions change and evolve across species but has been hindered by the lack of high-resolution genomic data.
Gut Microbiome in Chronic Pancreatitis
Published:
- As a Master’s student ready to begin my thesis I was motivated to continue working on a project that would utilize a cutting-edge technology to study the gut microbiome which I was very excited about.
- This excitement being contagious, I managed to convince my thesis supervisor to invest in a Nanopore sequencer for the lab.
- I used this opportunity to optimize protocls and setup the Nanopore sequencer in the lab and developed my bioinformatic skills starting with setting up a Linux workstation for the lab and implementing a bioinformatic pipeline for metagenomics analysis.
- I developed an end-to-end sampling and sequencing pipeline for metagenomics analysis of fecal samples using Nanopore sequencing in collaboration with a nearby hospital.
- In collaboration with a gastroenterologist we analyzed the human gut microbiome in chronic pancreatitis patients and compared them with healthy samples to identify disease-related changes in the gut microbiome.
Gut Metaproteomics
Published:
- As a undergraduate student, I was excited to work on a project that would utilize cutting-edge technology to study the gut microbiome.
- I surveyed and tested various approaches for sample preparation and metaproteomic analysis of fecal samples using LC-MS/MS.
- Successfully detected proteins of microbiome origin but concluded that higher detection power eg. using a nano-LC MS/MS would be needed for fecal metaproteomics.
iGEM Project - Cellfiefuge
Published:
- The iGEM (International Genetically Engineered Machines) competition is an annual, worldwide synthetic biology event that brings together students from diverse backgrounds to design and build genetically engineered systems using standard biological parts. In 2016, I co-initiated the first iGEM team at the Indian Institute of Science (IISc), Bengaluru, India.
- Our iGEM project aimed to cut infrastructure requirements for biomanufacturing initiatives by engineering bacteria that can autoinduce protein overexpression and autoaggregate when production is complete, replacing the need for centrifugation of large cultures.
- More in our poster summarizing the project: iGEM Project Poster
- An article summarizing our experience as the first team from the institute and how our efforts paved the way to future teams that have since won awards and special mentions for their work can be found here
Priority Effects in the honeybee gut
Published:
In this ongoing study we aim to understand how bacterial strains in the gut microbiome interact with each other. I assemble synthetic microbial communities comprising honeybee-associated Lactobacillus and Bifidobacterium species and feed them to honeybees to study how strains of the same species compete with each other. We expect to see priority effects at the strain-level and that species will coexist by niche partitioning. We have developed a high-throughput compatible PacBio metagenomic approach to quantitatively detect strains in the gut. A pilot experiment points to clear signs of co-existance of strains between species but priority effects between strains of the same species. I carried out more replicates and combinations of strains and am currently analyzing the data to confirm the observations from the pilot experiment.
Gut microbiome of wild and managed honeybees
Published:
Little is known about the gut microbiome of wild honeybees. Taking advantage of a large collection of wild honeybees of various subspecies from across Africa, painstakingly put together by our collaborators, we are investigating the differences in the gut microbiome of wild and managed honeybees. We are using shotgun metagenomics to resolve strain-level differences in the gut bacterial community and are currently analyzing the data to identify bacterial strains and species that are found in wild and managed honeybees and investigating their distribution across colonies of different subspecies.